This page is for the use of students who are taking BMS 631
2024 Notes - Location: Vet Path Research Bldg 119
This is a 2 credit course that is designed to bring a student up to date on the technology of flow cytometry. The course will be lecture based and will require students to give a short seminar at the conclusion of the semester which will be part of the final grade. The course starts with the historical discovery of the technology and will move to discussing each of the individual components - optics, electronics, fluidics, data processing and analysis. In addition, we will cover all of the current applications. At the conclusion of the course, students will have an excellent understanding of the field and will be capable of reading, interpreting and carrying out studies using this technology.
Purdue University Cytometry Laboratories
NOTE: The distribution of these materials is restricted without specific permission of Professor Robinson. No materials may be released to any publication organization, nor may these materials be distributed in any form without specific written permission of Professor Robinson. Specifically, no permission is given to reproduce any of these materials for courses outside not-for-profit educational institutions and permission should be requested and credit included when used in this instance. We normally give permission for use of these materials in academic institutions.If you submit these to course hero, course hero has been notified that anyone submitting these lectures must be reported back to Purdue University.
Class text is "Practical Flow Cytometry", 4th ed (Shapiro) you can download a free copy here or buy on ebay/Amazon
Here is an outline of the topics in the course.
Here is the Introduction to Watson's Book - worth a read
DateOlder lecturesLectFile (incl older lectures)File Size LinkJan 8
2024 Lect #1a&b PPT
Note: Jan 15 is MLK day.
Intro history &flow principPt12019
JPRJan 22
Concluding Lecture 1
Who,Why of Flow Cytometry (Pt 2-2020 On Line class
JPR
Jan 29
Here is the PPT
2023-3PPT
Light, Matter, and the basics of scatter
Recorded 2020 lecture is here.
JPRFeb 5
Online lecture only today Lect 3
(Light and matter)
Here is the PPT
2023-4PPT
JPR
Feb 12
Here is the PPT
JPR Feb 19
Recorded lecture only today
Here is the PPT
Optical Filters 2023 Video From Sl27 only
2023-6Video
- Detecton Systems 2019
JPRMar 4
Video Lect
Optical Filters
2019 Fluidic Systems
JPR
Mar 11
Spring break
+ Lecture 12 DNA-RNA cytometry
JPRMarch 18
Light Sources
Fluidics
Lect8Video2023
Fluidic systems
2023 Video here2023
Mar25
Video
ONLY
ONLINE LECTURE ONLY
April 1
Data structure
Lect10Video2023
JPR
Apr 8
Cell Sorting
Lect11Video 2023
2019 Cell Sorting
JPR April 15class Presentations
April 24
No class
May 4 end term Final Exam Take homeJPRLecturer: JPR: J. Paul Robinson (contact by email: jpr#flowcyt.cyto.purdue.edu)
Seminars should be 10-15 minutes and must not exceed 20 minutes, you must provide the PPT overview to me at minimum 2 weeks prior to review. I suggest that as soon as you have a topic and some preliminar notes, you discuss this with me so that I can guide you down the best pathway. If I have not reviewed and agreed to your presentation materials, you will automatically lose 50% of the points for this seminar. The whole purpose is to ensure that you present a high quality seminar relevent to the topis, and that your materials are accurate. A copy of your final PPT will be retained and we reserve the right to post this on the web with other seminars if we believe it would be useful to the scientific public. I will not review a seminar with 48 hours of your presentation, unless it has already been given to me for reveiw on at least one previous occasion.
NameDateExample Topics (must be related to flow cytometry and cannot be your research project - some examples are below) Fluorescent probes for Oxidative MetabolismSD03/15Monoclonal antibodies, Avidin-Biotin technology using Fluorescent Conjugates in Flow cytometry Principles of Sorting in Flow Cytometry.XW03/15Intracellular cytokine determination via flow cytometrySH03.19Optical Filters used in Flow CytometryKK03/15Cell proliferation assessed by flow cytometry using cell tracking dyesPH03/16Data analysis: How and why? Fluidics Systems in Flow Cytometry The principles of spectal overlapRU03/16Cytof- Mass CytometryCL03/15Fluorescent probes for apoptosisPS03/16Parameters and probes for evaluation of DNA &RNA in Flow Cytometry
All materials copyright © J. Paul Robinson 1989-2024 and may not be reproduced for commercial purposes. These slides may be used for educational use only by non-profit institutions and credits must be included for all slides used. In all cases, please request permission for use of these materials. No use is allowed for commercial purposes or for commerical courses of any nature.