TITLE: Research Associate I, Flow Cytometry. Exempt
REPORTING LOCATION: 1330 Piccard Dr, Rockville, MD 20850
REPORTS TO: Associate Manager, Flow Cytometry
PRIMARY OBJECTIVES: Support the pre-clinical and clinical teams.
DUTIES AND RESPONSIBILITIES
· Preparation and handling of primary specimens, including peripheral blood, ascites, other liquid biospecimens.
· Isolation of white blood cells by density gradient separation
· Primary immune cell culture (experience with T cells, NK cells, Dendritic cells, Macrophages)
· Sample preparation (murine or human tissues/cells) for immunostaining and acquisition by flow cytometry
· Cell culture for primary cells and cell lines
· Perform multicolor flow cytometry including autonomous use of flow cytometer(s) and flow analysis software
· Perform ex vivo and in vitro assays for immuno-oncology relevant endpoints (e.g. ELISA and multiplex cytokine assays)
· Troubleshoot, optimize, and develop methods for unmet experimental needs
· Maintain laboratory records and notebook, organize and compile data, and write weekly reports on results
KNOWLEDGE, SKILLS, AND ABILITIES
· Expertise in aseptic techniques for handling primary human cells and for mammalian cell culture
· Proficiency with processing primary tissues and other samples for downstream assays
· Proficiency in Immunostaining, flow Cytometry acquisition and analysis
· Good documentation practices and able to follow established standard operating procedures (SOPs)
· Experience working in a regulated environment (GLP or GCLP)
· Proficiency in MS office suite, including Excel, Word and PowerPoint
· Ability to coordinate parallel tasks across multiple projects, demonstrating prioritization.
EDUCATION AND EXPERIENCE
· BS with 2-year experience. Preferred field of study in immunology or related sciences.
· Industry experience working with pre-clinical and clinical samples is preferred.
· Experience with multicolor flow cytometry is required
PHYSICAL DEMANDS AND WORK ENVIRONMENT
· Must be able to sit and use hands to finger, handle and feel.
· Must be able to lift and move packages weighing up to 50 pounds.
· Must be able to sit for long periods of time using a computer in a typical office environment in a multi-level facility
· Some activities require repetitive motion and reaching, pulling or pushing.
· In carrying out the duties of the job there will be frequent exposure to fumes and chemical hazards.
· Duties performed routinely require exposure to blood, body fluid and tissue.
This job description may be changed to include new responsibilities and tasks or change existing ones as management deems necessary.