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BMS 631 Flow Cytometry: Theory (2 CR Course) - 2016 Course Notes

This page is for the use of students who are taking BMS 631

2016 Notes - Location: Vet Path Research Bldg 119

This is a 2 credit course that is designed to bring a student up to date on the technology of flow cytometry. The course will be lecture based and will require students to give a short seminar at the conclusion of the semester which will be part of the final grade. The course starts with the historical discovery of the technology and will move to discussing each of the individual components - optics, electronics, fluidics, data processing and analysis. In addition, we will cover all of the current applications. At the conclusion of the course, students will have an excellent understanding of the field and will be capable of reading, interpreting and carrying out studies using this technology.

 

Purdue University Cytometry Laboratories

NOTE: The use of these materials is restricted without specific permission of Professor Robinson. No materials may be released to any publication organization, nor may these materials be distributed in any form without specific written permission of Professor Robinson. Specifically, no permission is given to reproduce any of these materials for courses outside not-for-profit educational institutions and permission should be requested and credit included when used in this instance. We normally give permission for use of these materials in academic institutions.If you submit these to course hero, course hero has been notified that anyone submitting these lectures must be reported back to Purdue University.

 

Also, please note that I have added a couple of e-lectures that highlight the introduction to flow cytometry. These are in the E-Lectures option one level previous to this page.

 

E-lectures (You can take a look at these lectures - they are designed as introductory lectures for those will no knowledge of flow cytometry.)

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2016 Class notes and lecture Scedule - BMS 631: Flow Cytometry: Theory

Class text is "Practical Flow Cytometry", 4th ed (Shapiro)  you can download a free copy here

DateLect #TitleLectFileFile Size Link
Jan 111Introduction, history & basic flow principles (Pt 1)JPR

Lecture0001.ppt

2014-160Meg

(Automated PPT) 2016 Lecture

55 Megupdated 2016
Jan 18 No lecture today as Martin Luther King Day   updated 2016
Jan 252The Who and Why of Flow Cytometry: History Cont. (Pt 2)JPR

Lecture002.ppt

NEW Video lect

from Jan 25, 2016

 Updated 2016
Feb 13Light, Matter, and the basics of scatterJPRLecture003.ppt2 Megupdated 2016
Feb 84Properties of fluorescenceJPR

Lecture004.ppt

 

NEW VIDEO LINK FOR 2016 Lecture

 

Updated

2016

Feb155Light sourcesJPR

Lecture  Video NEW Link to recording 2016

Lecture 5 link

 updated 2016
Feb226Properties of optical filters in flow cytomeryJPRLecture 6 Updated 2016
Feb29

7

 

Detectors in flow cytometryJPR

Lecture007.pptx

 

 updated 2016
Mar 78

Fluidic Systems and cell sorting

JPRLecture008.pptx updated 2016
Mar11Lab tutorial at 1 pm In Lynn G221  
Mar21 No Lecture Spring break  
Mar2810Multiparameter Data Analysis (Lect 10) PLUS DNA-RNA probe (lecture 11)JPR

Lecture0010.ppt &

 Lecture0011.ppt

 Updated 2016

Apr 1

(1pm)

11

Cell Sorting /Cell function 

 Lecture0012.ppt

 

20 Meg

 

Apr1112Cell Function & CytometryJPRLecture 13.ppt  
Apr1813 JPR 3.4 meg   
Apr2514Microbiolgy & CytometryJPR Lecture 14.ppt4.8 Meg 
       
       
Final Exam Not yet setJPR   

Lecturer: JPR: J. Paul Robinson (contact by email: jpr#flowcyt.cyto.purdue.edu)

 

 

Seminars should be 15 minutes and must not exceed 20 minutes, you must provide the PPT overview to me at minimum 2 weeks prior to review. I suggest that as soon as you have a topic and some preliminar notes, you discuss this with me so that I can guide you down the best pathway. If I have not reviewed and agreed to your presentation materials, you will automatically lose 50% of the points for this seminar. The whole purpose is to ensure that you present a high quality seminar relevent to the topis, and that your materials are accurate. A copy of your final PPT will be retained and we reserve the right to post this on the web with other seminars if we believe it would be useful to the scientific public. I will not review a seminar with 48 hours of your presentation, unless it has already been given to me for reveiw on at least one previous occasion.

 

NameDateExample Topics (these are in order of topic selection -once selected these topics are not available)
  Fluorescent probes for Oxidative Metabolism
  Monoclonal antibodies, Avidin-Biotin technology using Fluorescent Conjugates in Flow cytometry
  Principles of Sorting in Flow Cytometry.
  Intracellular cytokine determination via flow cytometry
  Optical Filters used in Flow Cytometry
  Cell proliferation assessed by flow cytometry using cell tracking dyes
  Data analysis: How and why?

 

All materials copyright © J. Paul Robinson 1989-2016 and may not be reproduced for commercial purposes. These slides may be used for educational use only by non-profit institutions and credits must be included for all slides used. In all cases, please request permission for use of these materials. No use is allowed for commercial purposes or for commerical courses of any nature.

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