This page is for the use of students who are taking BMS 631
2016 Notes - Location: Vet Path Research Bldg 119
This is a 2 credit course that is designed to bring a student up to date on the technology of flow cytometry. The course will be lecture based and will require students to give a short seminar at the conclusion of the semester which will be part of the final grade. The course starts with the historical discovery of the technology and will move to discussing each of the individual components - optics, electronics, fluidics, data processing and analysis. In addition, we will cover all of the current applications. At the conclusion of the course, students will have an excellent understanding of the field and will be capable of reading, interpreting and carrying out studies using this technology.
Purdue University Cytometry Laboratories
NOTE: The use of these materials is restricted without specific permission of Professor Robinson. No materials may be released to any publication organization, nor may these materials be distributed in any form without specific written permission of Professor Robinson. Specifically, no permission is given to reproduce any of these materials for courses outside not-for-profit educational institutions and permission should be requested and credit included when used in this instance. We normally give permission for use of these materials in academic institutions.If you submit these to course hero, course hero has been notified that anyone submitting these lectures must be reported back to Purdue University.
Also, please note that I have added a couple of e-lectures that highlight the introduction to flow cytometry. These are in the E-Lectures option one level previous to this page.
E-lectures (You can take a look at these lectures - they are designed as introductory lectures for those will no knowledge of flow cytometry.)
2016 Class notes and lecture Scedule - BMS 631: Flow Cytometry: Theory
Class text is "Practical Flow Cytometry", 4th ed (Shapiro) you can download a free copy here
|Date||Lect #||Title||Lect||File||File Size||Link|
|Jan 11||1||Introduction, history & basic flow principles (Pt 1)||JPR|
(Automated PPT) 2016 Lecture
|55 Meg||updated 2016|
|Jan 18||No lecture today as Martin Luther King Day||updated 2016|
|Jan 25||2||The Who and Why of Flow Cytometry: History Cont. (Pt 2)||JPR|
NEW Video lect
|Feb 1||3||Light, Matter, and the basics of scatter||JPR||Lecture003.ppt||2 Meg||updated 2016|
|Feb 8||4||Properties of fluorescence||JPR|
|Feb15||5||Light sources||JPR||updated 2016|
|Feb22||6||Properties of optical filters in flow cytomery||JPR||Lecture 6||Updated 2016|
|Detectors in flow cytometry||JPR|
Fluidic Systems and cell sorting
|Mar11||9||Lab tutorial at 1 pm||In Lynn G221|
|Mar21||No Lecture||Spring break|
|Mar28||10||Multiparameter Data Analysis (Lect 10) PLUS DNA-RNA probe (lecture 11)||JPR||Updated 2016|
|Cell Sorting /Cell function|
|Apr11||12||Cell Function & Cytometry||JPR||Lecture 13.ppt|
|Apr25||14||Microbiolgy & Cytometry||JPR||Lecture 14.ppt||4.8 Meg|
|Final Exam||Not yet set||JPR|
Lecturer: JPR: J. Paul Robinson (contact by email: jpr#flowcyt.cyto.purdue.edu)
Seminars should be 15 minutes and must not exceed 20 minutes, you must provide the PPT overview to me at minimum 2 weeks prior to review. I suggest that as soon as you have a topic and some preliminar notes, you discuss this with me so that I can guide you down the best pathway. If I have not reviewed and agreed to your presentation materials, you will automatically lose 50% of the points for this seminar. The whole purpose is to ensure that you present a high quality seminar relevent to the topis, and that your materials are accurate. A copy of your final PPT will be retained and we reserve the right to post this on the web with other seminars if we believe it would be useful to the scientific public. I will not review a seminar with 48 hours of your presentation, unless it has already been given to me for reveiw on at least one previous occasion.
|Name||Date||Example Topics (these are in order of topic selection -once selected these topics are not available)|
|Fluorescent probes for Oxidative Metabolism|
|Monoclonal antibodies, Avidin-Biotin technology using Fluorescent Conjugates in Flow cytometry|
|Principles of Sorting in Flow Cytometry.|
|Intracellular cytokine determination via flow cytometry|
|Optical Filters used in Flow Cytometry|
|Cell proliferation assessed by flow cytometry using cell tracking dyes|
|Data analysis: How and why?|
All materials copyright © J. Paul Robinson 1989-2016 and may not be reproduced for commercial purposes. These slides may be used for educational use only by non-profit institutions and credits must be included for all slides used. In all cases, please request permission for use of these materials. No use is allowed for commercial purposes or for commerical courses of any nature.