Susan A. Ludmann, M.S.
10838 NE 148th Lane B2
Bothell, WA 98011
I am a highly skilled individual with 10 years postgraduate work experience in academic and biotechnology environments seeking challenging and rewarding employment with a biotechnology or biopharmaceutical company.
1991-1993 MIAMI UNIVERSITY Oxford, Ohio
Master of Science, Zoology
1988-1991 BOWLING GREEN STATE UNIVERSITY Bowling Green, Ohio
November 2005 – November 21, 2007 NASTECH PHARMACEUTICAL COMPANY
- Screened fluorescently labeled siRNAs for cellular uptake in a variety of cell types using Fluorescently Activated Cell Sorting (FACS) and fluorescent microscopy.
- Experience in staining and acquiring data using an FC500 Flow Cytometer (Beckman Coulter).
- Developed flow cytometric assays to assess the endocytic pathways used for uptake of siRNAs and other formulations of interest.
- Attended 29th Annual Course in Cytometry – Research Methods and Applications. Bowdoin College, Brunswick, Maine
- Screen compounds for permeability of high molecular weight molecules using an epiairway tissue model. Assay employed include TEER reduction, MTT, LDH release and ELISAs
- Cotransfections of plasmid and siRNAs for detection using a Dual Luciferase Reporter system
- Deconvolution Fluorescent microscopy of epiairway tissue (multiple cell layers thick)
1997- October, 2005 ChromaVision Medical Systems (now CLARiENT, INC.)
San Juan Capistrano, CA
- Reported directly to the Chief Science Officer and as the first laboratory employee hired at this start-up company had a strong influence on the development of the laboratory.
- Creative researcher capable of conceiving, designing and carrying-out experiments as well as organizing and presenting data allowing for rapid advancement of scientific projects.
- Experience with HUVEC primary cell lines as well as various tumor cell lines.
- Experience in staining and acquiring data using FACS AriaTM to detect rare cells in the peripheral blood of cancer patients.
- Magnetic enrichment of rare cells of interest from peripheral blood and bone marrow using the Miltenyi system.
- Development of cell preparation techniques as well as immunocytochemical staining procedures to work optimally with Clarient’s Automated Cellular Imaging System (ACISTM).
- Work has led to clinical collaborations with biopharmaceutical companies as well as prestigious medical institutions and academic groups.
- Actively involved in management of Phase I and Phase II clinical trials being carried out by Clarient’s BioAnalytical Services Group.
- Experience with writing SOPs and working in a GLP environment.
- Computer software skills include Photoshop 7.0, Exel and Powerpoint.
1993-1997 UNIVERSITY OF MINNESOTA St.Paul, MN
Department of Genetics & Cell Biology
Junior Scientist (1995-1997)
Senior Laboratory Technician (1993-1995)
- Immunofluorescence staining of mutant Drosophila ovaries with antibodies to various cytoskeletal proteins and subsequent confocal microscopic analysis.
- Prepared and screened northern blots with P1 plasmids in an attempt to clone a gene involved in the organization of the actin cytoskeleton in Drosophila oogenesis.
- Genetic analysis of EMS induced mutations in the cytoplasmic dynein heavy chain gene, as well as determining various loci which interact with the cytoplasmic dynein heavy chain gene (in particular the glued locus and kinesin heavy chain locus).
- PCR and initial cloning of Drosophila gene sequences homologous to genes in other organisms which encode intermediate and light chain polypeptides in the dynein complex.
1991-1993 MIAMI UNIVERSITY Oxford, OH
- Conducted a biochemical and functional analysis of a motility mutant in Tetrahymena thermophila lacking flagellar outer arm dyneins.
- Work included running 2-D (IEF) gels and Transmission Electron Microscopy.
- Responsible for instructing General Biology and Developmental Biology laboratories.
1989-1991 BOWLING GREEN STATE UNIVERSITY Bowling Green, OH
Laboratory Technician (May, 1991 - August, 1991)
Undergraduate Research Assistant (August, 1989-May, 1991)
- Responsible for screening Drosophila melanogaster which had undergone a local duplication of a P transposable element for maternal effect mutations.
- Conducted genetic crosses designed to eliminate a lethal mutation from a chromosome carrying a female-sterile mutation.
- Conducted a northern blot analysis of female-sterile Drosophila to determine if a maternal- specific sequence corresponded to any of the female-sterile loci in the chromosomal region.
1. Ramasubban, S., Heller, E., Kowal-Vern, A., Webster, S., Ludmann, S., Bauer, K. and Rubin, D. 2004. Circulating Endothelial Cells in Patients with Acute Lung Injury. American Journal of Respiratory and Critical Care Medicine. Recently accepted.
2. Bauer, K.D., de la Torre-Bueno, J., Diel, I.J., Hawes, D., Decker, W.J., Priddy, C., Bossy, B., Ludmann, S.A., Yamamoto, K., Masih, A.S., Espinoza, F.P. and Harrington, D. 2000. Reliable and Sensitive Analysis of Occult Bone Marrow Metastases Using Automated Cellular Imaging. Clinical Cancer Research. 6:3552-3559.
3. Li, M-g., Serr, M., Edwards, K., Ludmann, S., Yamamoto, D., Tilney, L.G., Field, C.M. and Hays, T. S. 1999. Filamin Is Required for Ring Canal Assembly and Actin Organization During Drosophila Oogenesis. The Journal of Cell Biology. 146:1061-1074
4. Gepner, J., Li, M-g., Ludmann, S., Kotas, C., Boylan, K., Iyadurai, S., McGrail, M. and Hays, T. 1996. Cytoplasmic Dynein Function is Essential in Drosophila Melanogaster. Genetics. 142:865-878.
5. Sullivan, J. L., Ludmann, S. A., Hamasaki, T. and Pennock, D. G. 1996. Analyses of 22S Dynein Binding to Tetrahymena Axonemes Lacking Outer Dynein Arms. Journal of Eukaryotic Microbiology. 43:5-11.
6. McGrail, M., Gepner, J., Silvanovich, A., Ludmann, S., Serr, M. and Hays, T. 1995. Regulation of Cytoplasmic Dynein Function In Vivo by the Drosophila Glued Complex. The Journal of Cell Biology. 131(2):411-425.
7. Ludmann, S. A., Schwandt, A., Kong, X., Bricker, C. S. and Pennock, D. G. 1993. Biochemical Analysis of a Mutant Tetrahymena Lacking Outer Dynein Arms. The Journal of Eukaryotic Microbiology. 40:650-660.
1. Witkowska, R.T., Ahmadian, M., Purvis, L.J., Roth, S., Ludmann, S., Wang, H., Dattilo, J.W., Farber, K.W., Mayer, S.J., Chen, L., Chen, Y., Cui, K., Houston, M.E., Quay, S.C. and Johnson, P. H. 2006. Peptide-Mediated Delivery of siRNA via Covalent Conjugates. 29th European Peptide Symposium. Gdansk, Poland.
2. Bauer, K., Webster, S., Ludmann, S., Torre-Bueno J., Bossy, B. 2003. Use of Automated Cellular Imaging for the Detection and Analysis of Occult Bone Marrow Metastasis. J. Cancer Res Clin Oncol 129:20.
3. Ludmann, S.A., Webster, S., Bauer, K. and Harrington, D.S. 2002. Development of a Model System to Detect Circulating Endothelial Cells in Peripheral Blood Coupled with Automated Cellular Imaging. XXI International Congress of the International Society for Analytical Cytology (ISAC).
4. Bossy, B., Ludmann, S.A., Webster, S., Bauer, K. and Harrington, D.S. 2002. Enhanced Detection of Occult Breast Carcinoma Cells Using Multiple-Antigen Immunomagnetic Selection. XXI International Congress of the International Society for Analytical Cytology (ISAC).
5. Webster, S., Ludmann, S.A., Bauer, K. and Harrington, D.S. 2002. Development of a Model System for the Detection of Circulating p24 Expressing Cells in HIV Positive Patients. XXI International Congress of the International Society for Analytical Cytology (ISAC).
6. Harrington, D.S., Bossy, B., Ludmann, S.A., Torre-Bueno, J. and Bauer, K. 2001. Rare Breast Carcinoma Cell Detection Can Be Enhanced Using Multiple-Antigen Immunomagnetic Capture In Combination With Automated Cellular Imaging. Proc: 3rd International Symposium on Minimal Residual Cancer.
7. Bossy, B., Ludmann, S.A., Torre-Bueno, J. Harrington, D.S., and Bauer, K. 2001. Enhancement of Rare Breast Carcinoma Cell Detection Using Multiple-Antigen Immunomagnetic Capture. Proc: Amer Assoc Cancer Res. 42:11.
8. Warraich I., Ludmann, S., Zhang, H., Aja T., Gladstone, P., Ching, B., Bauer, K., Harrington, D., Terrand, J. 2001. CMV pp65 Antigenicity Enhancement by Antiapoptotic Agent IDN7312 with Automated Cellular Imaging Analysis. Lab Invest. 81(1):237a.
9. Harrington, D.S., Terrand, J.J., Torre-Bueno, J., Ludmann, S., Yamamoto, K., Zhang, H. and Bauer, K.D. 2000. Reliable and Sensitive Analysis of Cytomegalovirus pp65 Antigenemia Using Automated Cellular Imaging. Modern Pathology. 13:233A.
10. Hawes, D., Masih, A., Espinosa, F., Ludmann, S., Watson, M., Harrington, D and Bauer, K. 1998. Automated Detection of Rare Carcinoma Cells in Bone Marrow Using the ChromaVision Automated Cellular Imaging System (ACIS). Proc: 2nd International Symposium on Minimal Residual Cancer.