NAME: Huw Stephen KRUGER GRAY.
Home address: Apartment 44, 370 Chestnut Hill Avenue, Brighton, MA, 02135-7779, U.S.A..
Telephone: (617)-975-2868 (answering machine when absent).
(617)-331-2870 (mobile ‘phone).
Electronic mail address: ZipKG@yahoo.com
SCIENTIFIC TRAINING & EXPERIENCE:-
· Field instrument technical & applications support, including system installations & user training, diagnosis & trouble-shooting, system up-grades, applications advice & consultation, instrument service, support for instrument sales & trials, etc. in many countries, including Africa and Haiti.
· Research, application, development & tuition of Flow Cytometry, with on‑line computer data acquisition, presentation, off-line data transfer, analysis & archiving.
· Bulk cell sterile (& high-speed) flow-sorting, four-way sorting & single cell flow-cloning.
· Flow cytometric techniques: many & varied, especially immuno-phenotyping (surface & cytoplasmic), D.N.A. study (cell-cycle, ploidy, side-population, etc.), apoptosis, et. al. & assorted combinations.
· Establishment, supervision, management & operation of sophisticated Flow Cytometry Core Facilities, including consultation, liaison, scheduling, budgeting & accounting, with direct involvement in many diverse research & clinical projects.
· Confocal Laser Scanning Fluorescence Microscopy & 3-D. Imaging.
· Field testing of novel reagents & prototype instrument systems.
· Computing (software & hardware; mainframe & micro‑computers.) & networking.
· Microbiological techniques, including continuous chemostat culture & fermentation (design, instrumentation, operation).
· Biochemical analyses.
· Applications for research and development grants, awards, etc..
· Writing and editing of books, papers, documents, brochures, grant applications, statements, etc..
· Lecturing to undergraduate & medical students; Setting, marking & grading term quizzes, final examinations & practical class reports; Demonstrations for U.K. school 'A' level biology teachers' refresher course, undergraduate practical classes & student tutorials.
· Help & supervision of instrument users, project students & technicians.
· Scientific presentations at international congresses, plus other symposia, seminars & meetings, publications in learned journals.
2006 - 2007: PointCare Technologies Inc., 181, Cedar Hill Street, Marlborough, MA, 01752, U.S.A..
Field Technical Support Manager, providing field technical and applications support for their clinical AuRICA cytometric human A.I.D.S. diagnostic CD4 analyser. This novel system uses colloidal gold micro-particles as a simple assay for CD4 on lymphocytes, detected by changes in side-scatter signals. The entire assay is performed within the fully enclosed instrument automatically and can be operated by relatively inexperienced technicians, such as often are found in Third World cuntries. Based just outside Boston and covering the entire globe, especially focusing upon sub-saharan Africa and the Caribbean. This involved substantial travel to countries including Kenya, Nigeria, Tanzania and Haiti, to provide technical support in multiple rural bush clinics and hospitals. Substantial involvement in the winning of a major World Bank award, for the development of a mobile laboratory vehicle, to provide travelling analytical services to H.I.V. patients in their rural bush communities in Malawi. Fist author of a S.B.I.R. grant application, to develop a novel simple cytometric assay for liver toxicity, often caused after prolonged anti-retroviral drug therapy.
2005-2006: Xcellerex Inc., 170 Locke Drive, Marlborough, MA, 01752, U.S.A..
Two month temporary consultant position in the Process Development group, running their G.M.P. compliant flow cytometry facility, using a FACScan analyser and a MoFlo high-speed cell sorter. Developing and refining protocols for contract sorting and cloning of producer cell lines.
2005: Biogen-Idec Inc., 14 Cambridge Centre, Cambridge, MA, 02142, U.S.A..
Five month temporary consultant position, in their busy core flow cytometry facility, housing three FACS Calibur analysers, a L.S.R. II multi-laser analyzer, a MoFlo high-speed cell sorter and a new Cytopeia InFlux multi-parameter cell sorter.
2005: Clinical Flow Cytometry Laboratory, Massachusetts General Hospital, Warren 1st. floor, 55 Fruit Street, Boston, MA, 02114, U.S.A..
Two month temporary consultant position, staining and analysing clinical samples, such as fresh blood from A.I.D.S. patients and those with various tumours, including leukaemia, lymphoma, etc. using FACS Calibur analysers.
2004-2005: Cytometry Facility, Dana-Faber Cancer Institute, J415, 44 Binney Street, Boston, MA, 02115, U.S.A..
Three month temporary consultant position, as acting director of their busy core flow cytometry facility, housing three FACScan analysers, an old FACS Vantage cell sorter and three multi-laser, multi-parameter MoFlo high-speed cell sorters.
2002-2004: Guava Technologies Inc., 25801, Industrial Boulevard, Hayward, CA, 94545, U.S.A..
Senior Technical Specialist, providing field technical and applications support for the P.C.A. (Personal Cell Analyser), P.C.A.-96 automated screening cytometer and P.C.A.-96-A.F.P. blue laser system. Based in Boston and covering the U.S. North Eastern territory, from New York City up to Canada and west to Buffalo. Guava is a biotechnology company, which develops, manufactures and markets patented, integrated cellular analysis systems, for the life sciences. The company is changing the way in which cell culture monitoring and cell screening assays are performed, by providing compact micro-volume scale systems that make common assays in cell-based analysis accessible directly at the bench. Guava’s products have broad applications to life sciences research, drug discovery and biopharmaceutical production, but also offer potential for use in clinical testing and blood processing, as well as other areas where simple, single cell analysis is important.
1997-2002: BioTransplant Inc., Building 75, Third Avenue, Charlestown Navy Yard, Charlestown, MA, 02129, U.S.A..
Scientist, to establish & manage a new core Flow Cytometry facility, serving the entire joint company’s requirements for flow cytometric analysis, cell sorting etc.. Instruments included a new custom-built Cytomation three laser (Argon, mixed gas & HeNe) MoFlo high-speed four-way cell sorter & Becton-Dickinson FACScan. Typically, these research instruments are used for cell cycle analysis, study of apoptosis, signalling, for measuring the reaction of monoclonal antibodies with complex mixtures of cells & for sorting in order to separate individual sub-populations from mixtures of cells.
1997: Biogen Inc., 14 Cambridge Centre, Cambridge, MA, 02142, U.S.A..
Temporary Consultant in Flow Cytometry, to manage the cytometry laboratory equipped with a Becton-Dickinson twin laser FACStar-Plus & two FACScans.
1997: Cytometry Facility, Dana-Faber Cancer Institute, J319A, 44 Binney Street, Boston, MA, 02115, U.S.A..
Wrote & delivered a series of lectures comprising an “Introduction to Flow Cytometry”, for users of the D.F.C.I. core flow cytometry facility.
1996-1997: Cytometry Laboratories, Veterinary Medicine School, Basic Medical Sciences Department, Purdue University, West Lafayette, Indiana, 47907-1515, U.S.A..
Seven month temporary appointment as post-doctoral Research Assistant, working on various projects involving laser scanning confocal imaging (Bio-Rad MRC 1024 & DVC 250) as an analytical tool, development of DNA assays, image & data analysis, computing & production of the third in a series of Cytometry CD-ROM disks (e.g. co-ordinator & H.T.M.L. code writer for two sections, author of three papers), plus the first Microscopy CD-ROM, as well as being involved extensively in the teaching programmes of the Cytometry Laboratory.
1995‑1996: Pathology Department, University of Texas Southwestern Medical Centre, Dallas, Texas, 75235-9072, U.S.A..
Post-doctoral Research Fellow on a one-year contract to run the core Flow Cytometry Facility, housing a B.‑D. FACStar-Plus three laser (488nm., U..V.. & pumped-dye) cell sorter, with an Automatic Cell Deposition Unit (A.C.D.U.), plus a FACScan cell analyser (also a prototype FACScalibur in another laboratory) for research applications, with Hewlett Packard 340, 310 & Apple Macintosh computers for off-line data analysis. Other instruments also within the facility, which were used exclusively for clinical applications, include a B.-D. FACSort & a Coulter Epics XL,
1994‑1995: Pathology Department, Tufts University Medical School, Boston, Massachusetts, 02111, U.S.A..
Research Assistant Professor on the Medical School Faculty, with a one-year contract as Chief of Operations of the Pathology Flow Cytometry Facility, housing a B.‑D. FACStar+ twin laser cell sorter with an A.C.D.U., plus a B.-D. FACScan analyser & a P.C. for off-line data analysis.
1986‑1994: Immunology Division, Pathology Department, Cambridge University, Tennis Court Road, Cambridge, CB2 1QP, England.
Post-doctoral Research Associate: "Applications of Flow Cytometry". In charge of the core flow cytometry laboratory, housing an Ortho Cytofluorograph 50‑H twin laser cell sorter, plus two B.‑D. FACScan analysers, each with FACSmate robots & four H.-P.340 computers linked with a FACSnet network.
1984‑1986: Various temporary positions, finishing as Records Assistant for the Suffolk Biological Records Centre, Ipswich, England, writing biological database software, using dBase- II on a PC.
1978‑1984: Department of Biology, University of Essex, Colchester, Essex, CO4 3SQ, England.
Degree awarded: Ph.D. Microbiology: 1985.
Project title: "The effect of acidity on the physiology of Arthrobacter globiformis", involving continuous chemostat fermentor culture of an important soil bacterium.
1975‑1978: School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, England.
Degree awarded: B.Sc. (Hons.) Biological Sciences: Class II(1): 1978.
Membership of Learned Societies:-
Chartered Biologist: Member of the Institute of Biology; International Society for Analytical Cytology; Society for General Microbiology; Royal Microscopical Society; British Society for Immunology.
National Flow Cytometry Resource, annual flow cytometry course: Los Alamos National Laboratories, New Mexico, USA: 2001.
Light aviation (both U.K. and U.S. Private Pilot’s Licences); Glider pilot (B.G.A. Bronze “C” certificate); Motor-cycles (owner of 1975 Norton 850 Commando and 2006 Legend chopper; local chapter Road Captain); S.C.U.B.A. diving (P.A.D.I. Advanced open-water and Nitrox certificates); Classic and sports cars (owner of 1982 Reliant Scimitar GTE); Photography; Nature and conservation; Fine wines and real ales (C.A.M.R.A. member); Music (rock and classical; amateur bass guitarist); Computers; Renaissance faires; Travel; Pets; Acting; Parachuting; Model making.
Available upon request.
Available upon request.