What can Flow Cytometry Do?

7/6/00


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Table of Contents

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Purdue University Cancer Center & Purdue University Cytometry Laboratories

What can Flow Cytometry Do?

Introductory Terms and Concepts

Concepts

Instrument Components

Arc Lamp Excitation Spectra

Lasers

Optical Collection systems

Elite Cytometer with 4 Lasers

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Coulter Optical System Elite/Altra

Fluidics

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Fluorescence

Fluorescence

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Fluorescence

Flow cytometry measurements

Data Presentation Formats

Data Analysis Concepts

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The Cell Cycle

A DNA histogram

A typical DNA Histogram

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Labeling Strand Breaks with dUTP [Fluorescein-deoxyuridine triphosphate (dUTP)]

Scatter Pattern of Human leukocytes

Three Color Lymphocyte Patterns

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Live cell/dead cell PI Fluorescence

Oxidative Reactions

Calcium Flux

Membrane Potential

Summary

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Author: Cytometry Labs

Email: jpr@flowcyt.cyto.purdue.edu

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